Researchers at the University of California, Irvine have developed a microfluidic device that has a combination of side wall and planar electrodes designed to generate magnetohydrodynamics (MHD) and dielectrophoresis (DEP) forces on cells in solution. The MHD and DEP forces can separate a heterogeneous population of cells based on their different dielectric properties and sizes.
The device comprises of a main channel with two side walls and a bottom surface. A plurality of vertically-oriented electrodes is disposed on the first wall and on the second wall opposite to the first wall. As cells flow down the channel containing the two side walls, the electrodes are used to generate DEP and MHD forces vertical to the main stream of the flow. Different cell populations will be subjected to different forces from the opposing electrode arrays affecting the separation of different cell populations and thus resulting in lateral separation of the cells. The cells then can be separated by DEP switching to direct them either into additional channels for further separation or wells for collection.
This device may be used to separate heterogeneous cell populations for research, diagnostic, and treatment purposes.
Cells can be separated laterally along the channel direction and then automatically directed to the downstream channel branches for collection or further separation. Unlike FACS, the cells are not required to be modified for separation.
Prototypes have been made.
Prototypes have been shown to separate different populations of cells.
|United States Of America||Issued Patent||7,964,078||06/21/2011||2006-426|
microfluidic, DEP, dielectrophoresis, magnetohydrodynamics, MHD, cell separation